Magnetic Beads:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime Cat#P0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Lysis:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime Cat#P0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Transfection:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime Cat#P0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Recombinant:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime Cat#P0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
CCK-8 Assay:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime Cat#P0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Bicinchoninic Acid Protein Assay:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime Cat#P0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Multiple Displacement Amplification:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime Cat#P0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Isolation:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime Cat#P0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Cell Culture:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime Cat#P0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Reporter Assay:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime Cat#P0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
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